The Laboratoire
mixte CNRS / INRA / Bayer CropScience has been created at the instigation
of both private and public partners to favor the development of novel
agronomic solutions by the reinforcement of the interactions and exchanges
between basic- and applied research. Since 15 years, the results that
have been obtained have led to the publication of more than 100 scientific
papers and of several patents, thereby strengthening competitiveness
of the company. Moreover the Laboratory mixte has acquired an internationally
recognized expertise in plant-, seed-, and fungus-physiology, biochemistry
and molecular biology. This knowledge and expertise has been applied
in the fields of herbicides, fungicides, seed treatments and agronomic
and quality traits. Because of the status of this laboratory, research
actions have always been planned in common, taking into account interest
of both public and private partners. This success is due to a volunteer
effort for developing pluri-disciplinar approaches involving researchers
of different cultures, acting on common projects. The laboratory is
located within CRLD (Centre de Recherche de la Dargoire, Lyon, France).
It operates in close contact with this research center and also with
the other BCS research centers. As example, the following recent projects
have successfully been carried out in common:
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- comparison of the metabolism of essential amino acids (branch-chain
and sulfur-containing amino acids) in fungi and plants toward the
characterization of specific enzyme inhibitors that can lead to the
design of chemicals acting either as specific herbicides or fungicides;
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- overexpression in crop plants of enzymes of the sulfur amino acid
pathway that play major role in plant tolerance against abiotic stresses;
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- protein structure/activity analysis allowing generation of key mutations
conferring a decrease of sensitivity to key herbicides and therefore
allowing the production of tolerant crops;
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- characterization of molecular markers of seed vigor that can be
used to improve the quality of commercial seed lots;
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- development of functional genomics, and participation in the implementation
of transcriptome analysis using the plant pathogenic fungus, Magnaporthe
grisea, in order to identify targets and mode of action of novel fungicides.
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Selected papers
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- Biou V, Dumas R, Pebay-Peyroula E, Cohen-Addad C, Job
D, Douce R (1997) Three-dimensional structure of acetohydroxyacid
isomeroreductase from spinach chloroplasts at 2.2 Å resolution:
Structural characterization of herbicide-conjugating plant acetohydroxyacid
isomeroreductase. EMBO J 16, 3405-3415
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- Ravanel S, Gakière B, Job D, Douce R (1998)
The specific features of methionine biosynthesis and metabolism
in plants. Proc Natl Acad Sci USA 95, 7805-7812
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- Droux M, Ruffet M-L, Douce R, Job D (1998) Interactions
between serine acetyl transferase and O-acetylserine(thiol) lyase
in higher plants: structural and kinetic properties of the free
and bound enzymes. Eur J Biochem 255, 235 – 245
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- Alban C, Job D, Douce R (2000) Biotin metabolism
in plants. Ann Rev Plant Physiol Plant Mol Biol 51, 17-47
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- Clergeot PH, Latorse MP, Laurans F, Pépin R, Tharreau
D, Notteghem JL, Lebrun MH (2001) PLS1, a gene encoding
a tetraspanin-like protein is required for penetration of host leaves
by the rice blast fungus Magnaporthe grisea. Proc Natl Acad Sci
USA 98, 6963-6968
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- Dumas R, Biou V, Halgand F, Douce R, Duggleby R (2001)
Enzymology, structure and dynamics of acetohydroxy acid isomeroreductase.
Accounts of Chemical Research 34, 399-408
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- Gakière B, Denis L, Droux M, Job D (2002) Overexpression
of cystathionine g-synthase in Arabidopsis
thaliana leads to increased levels of methionine and S-methylmethionine.
Plant Physiol Biochem 40, 119-126
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- Denis L, Grossemy M, Douce R, Alban C (2002) Molecular
characterization of a second copy of holocarboxylase synthetase
gene (hcs2) in Arabidopsis thaliana. J Biol Chem 277, 10435-10444
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- Tabe LM, Droux M (2002) Limits to sulfur accumulation
in transgenic lupin seeds expressing a foreign sulfur-rich protein.
Plant Physiol 128, 1137-1148
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- Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerckhove
J, Job D (2002) Proteomics of Arabidopsis seed germination.
A comparative study of wild type and gibberellin deficient seeds.
Plant Physiol 129, 823-837
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- Gourgues M, Clergeot PH, Veneault C, Cots J, Sibuet S,
Brunet-Simon A, Levis C, Langin T, Lebrun MH (2002) A new
class of tetraspanins in fungi. Biochem Biophys Res Commun 297,
1197-204.
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- Picciocchi A, Douce R, Alban C (2003) The plant
biotin synthase reaction. Identification and characterization of essential
mitochondrial accessory protein components. J Biol Chem 278, 24966-24975
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- Droux M (2004) Sulfur assimilation and the role
of sulfur in plant metabolism: a survey.
Photosynth Res. 79, 331-348
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- Bohnert HU, Fudal I, Dioh W, Tharreau D, Notteghem JL,
Lebrun MH (2004) A putative polyketide synthase/peptide synthetase
from Magnaporthe grisea signals pathogen attack to resistant
rice. Plant Cell 16, 2499-2513.
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- Rajjou L, Gallardo K, Debeaujon I, Vandekerckhove J, Job
C, Job D (2004) The effect of alpha-amanitin on the Arabidopsis
seed proteome highlights the distinct roles of stored and neosynthesized
mRNAs during germination. Plant Physiol 134, 1598-1613
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- Fudal I, Bohnert HU, Tharreau D, Lebrun MH (2005)
Transposition of MINE, a composite retrotransposon, in the avirulence
gene ACE1 of the rice blast fungus Magnaporthe grisea. Fungal
Genet Biol 42, 761-772.
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- Job C, Rajjou L, Lovigny Y, Belghazi M, Job D (2005) Patterns
of Protein Oxidation in Arabidopsis Seeds and during Germination.
Plant Physiol138, 790-802
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- Veneault-Fourrey C, Lambou K, Lebrun MH (2006)
Fungal Pls1 tetraspanins as key factors of penetration into host plants:
a role in re-establishing polarized growth in the appressorium? FEMS
Microbiol Lett 256, 179-184.
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- Rajjou L, Belghazi M, Huguet R, Robin C, Moreau A, Job C,
Job D (2006) Proteomic investigation of the effect of salicylic
acid on Arabidopsis seed germination and establishment of early defense
mechanisms. Plant Physiol 141, 910-923
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