The Laboratoire mixte CNRS / Bayer CropScience

          The Laboratoire mixte CNRS / INRA / Bayer CropScience has been created at the instigation of both private and public partners to favor the development of novel agronomic solutions by the reinforcement of the interactions and exchanges between basic- and applied research. Since 15 years, the results that have been obtained have led to the publication of more than 100 scientific papers and of several patents, thereby strengthening competitiveness of the company. Moreover the Laboratory mixte has acquired an internationally recognized expertise in plant-, seed-, and fungus-physiology, biochemistry and molecular biology. This knowledge and expertise has been applied in the fields of herbicides, fungicides, seed treatments and agronomic and quality traits. Because of the status of this laboratory, research actions have always been planned in common, taking into account interest of both public and private partners. This success is due to a volunteer effort for developing pluri-disciplinar approaches involving researchers of different cultures, acting on common projects. The laboratory is located within CRLD (Centre de Recherche de la Dargoire, Lyon, France). It operates in close contact with this research center and also with the other BCS research centers. As example, the following recent projects have successfully been carried out in common:

  • comparison of the metabolism of essential amino acids (branch-chain and sulfur-containing amino acids) in fungi and plants toward the characterization of specific enzyme inhibitors that can lead to the design of chemicals acting either as specific herbicides or fungicides;
  • overexpression in crop plants of enzymes of the sulfur amino acid pathway that play major role in plant tolerance against abiotic stresses;
  • protein structure/activity analysis allowing generation of key mutations conferring a decrease of sensitivity to key herbicides and therefore allowing the production of tolerant crops;
  • characterization of molecular markers of seed vigor that can be used to improve the quality of commercial seed lots;
  • development of functional genomics, and participation in the implementation of transcriptome analysis using the plant pathogenic fungus, Magnaporthe grisea, in order to identify targets and mode of action of novel fungicides.
Selected papers

  • Biou V, Dumas R, Pebay-Peyroula E, Cohen-Addad C, Job D, Douce R (1997) Three-dimensional structure of acetohydroxyacid isomeroreductase from spinach chloroplasts at 2.2 Å resolution: Structural characterization of herbicide-conjugating plant acetohydroxyacid isomeroreductase. EMBO J 16, 3405-3415
  • Ravanel S, Gakière B, Job D, Douce R (1998) The specific features of methionine biosynthesis and metabolism in plants. Proc Natl Acad Sci USA 95, 7805-7812
  • Droux M, Ruffet M-L, Douce R, Job D (1998) Interactions between serine acetyl transferase and O-acetylserine(thiol) lyase in higher plants: structural and kinetic properties of the free and bound enzymes. Eur J Biochem 255, 235 – 245
  • Alban C, Job D, Douce R (2000) Biotin metabolism in plants. Ann Rev Plant Physiol Plant Mol Biol 51, 17-47
  • Clergeot PH, Latorse MP, Laurans F, Pépin R, Tharreau D, Notteghem JL, Lebrun MH (2001) PLS1, a gene encoding a tetraspanin-like protein is required for penetration of host leaves by the rice blast fungus Magnaporthe grisea. Proc Natl Acad Sci USA 98, 6963-6968
  • Dumas R, Biou V, Halgand F, Douce R, Duggleby R (2001) Enzymology, structure and dynamics of acetohydroxy acid isomeroreductase. Accounts of Chemical Research 34, 399-408
  • Gakière B, Denis L, Droux M, Job D (2002) Overexpression of cystathionine g-synthase in Arabidopsis thaliana leads to increased levels of methionine and S-methylmethionine. Plant Physiol Biochem 40, 119-126
  • Denis L, Grossemy M, Douce R, Alban C (2002) Molecular characterization of a second copy of holocarboxylase synthetase gene (hcs2) in Arabidopsis thaliana. J Biol Chem 277, 10435-10444
  • Tabe LM, Droux M (2002) Limits to sulfur accumulation in transgenic lupin seeds expressing a foreign sulfur-rich protein. Plant Physiol 128, 1137-1148
  • Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerckhove J, Job D (2002) Proteomics of Arabidopsis seed germination. A comparative study of wild type and gibberellin deficient seeds. Plant Physiol 129, 823-837
  • Gourgues M, Clergeot PH, Veneault C, Cots J, Sibuet S, Brunet-Simon A, Levis C, Langin T, Lebrun MH (2002) A new class of tetraspanins in fungi. Biochem Biophys Res Commun 297, 1197-204.
  • Picciocchi A, Douce R, Alban C (2003) The plant biotin synthase reaction. Identification and characterization of essential mitochondrial accessory protein components. J Biol Chem 278, 24966-24975
  • Droux M (2004) Sulfur assimilation and the role of sulfur in plant metabolism: a survey.
    Photosynth Res. 79, 331-348
  • Bohnert HU, Fudal I, Dioh W, Tharreau D, Notteghem JL, Lebrun MH (2004) A putative polyketide synthase/peptide synthetase from Magnaporthe grisea signals pathogen attack to resistant rice. Plant Cell 16, 2499-2513.
  • Rajjou L, Gallardo K, Debeaujon I, Vandekerckhove J, Job C, Job D (2004) The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination. Plant Physiol 134, 1598-1613
  • Fudal I, Bohnert HU, Tharreau D, Lebrun MH (2005) Transposition of MINE, a composite retrotransposon, in the avirulence gene ACE1 of the rice blast fungus Magnaporthe grisea. Fungal Genet Biol 42, 761-772.
  • Job C, Rajjou L, Lovigny Y, Belghazi M, Job D (2005) Patterns of Protein Oxidation in Arabidopsis Seeds and during Germination. Plant Physiol138, 790-802
  • Veneault-Fourrey C, Lambou K, Lebrun MH (2006) Fungal Pls1 tetraspanins as key factors of penetration into host plants: a role in re-establishing polarized growth in the appressorium? FEMS Microbiol Lett 256, 179-184.
  • Rajjou L, Belghazi M, Huguet R, Robin C, Moreau A, Job C, Job D (2006) Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms. Plant Physiol 141, 910-923