In our
experiments non-dormant seeds of Arabidopsis, ecotype Landsberg erecta
(Ler), are referred to as wild type seeds. Germination assays are
carried out on three replicates of 25-64 seeds.
Seeds
were incubated at 25°C, with 8-h light daily, on three sheets
of absorbent paper (Roundfilter paper circles, Ø 45 mm, Schleicher
& Schuell, Dassel, Germany) and a black membrane filter with a
white grid (ME 25/31, Ø 45 mm, Schleicher & Schuell, Dassel,
Germany) wetted with 1.3 ml of distilled water, in covered plastic
boxes (Ø 50 mm). A seed is regarded as germinated when the
radicle protruded through the seed coat.
