Tools
Proteins
Contacts

Germination assays

          
           In our experiments non-dormant seeds of Arabidopsis, ecotype Landsberg erecta (Ler), are referred to as wild type seeds. Germination assays are carried out on three replicates of 25-64 seeds.
          Seeds were incubated at 25°C, with 8-h light daily, on three sheets of absorbent paper (Roundfilter paper circles, Ø 45 mm, Schleicher & Schuell, Dassel, Germany) and a black membrane filter with a white grid (ME 25/31, Ø 45 mm, Schleicher & Schuell, Dassel, Germany) wetted with 1.3 ml of distilled water, in covered plastic boxes (Ø 50 mm). A seed is regarded as germinated when the radicle protruded through the seed coat.